In addition to drug compounds, there are a large number of endocrine disrupting chemicals (EDC) in our environment, which pose a serious threat to living organisms. Among vertebrates, the role of fish in environmental risk assessment is outstanding, and it has long been used for toxicological testing as well. Tg(vtg1:mCherry) zebrafish, in addition to the classic toxicological endpoints and symptoms, indicates the EDC involvement of the tested samples with a clear “red light”. There are many benefits to using a transgenic line: